PCR Cycle Calculator


PCR (Polymerase Chain Reaction) is a crucial technique in molecular biology, allowing the amplification of DNA sequences. To streamline the process, a PCR cycle calculator becomes an indispensable tool. In this article, we’ll provide you with a user-friendly calculator,along with a detailed explanation of its functionalities.

How to Use

The PCR cycle calculator is designed for simplicity. Follow these steps to make accurate calculations:

  1. Input the initial DNA concentration in nanograms (ng).
  2. Enter the number of cycles desired for your PCR reaction.
  3. Input the efficiency percentage of your PCR reaction.
  4. Click the “Calculate” button to obtain the final DNA concentration after the specified cycles.


The formula used in the calculator is based on the exponential growth equation:

Example Solve

Suppose you start with an initial DNA concentration of 10 ng, plan 30 cycles, and your PCR reaction has an efficiency of 90%.

The calculated result will give you the DNA concentration after 30 cycles.


Q1: How accurate is the PCR cycle calculator?

A: The calculator uses the precise exponential growth equation for PCR, ensuring accurate results for your experiments.

Q2: Can I use this calculator for different PCR reactions?

A: Yes, the calculator is versatile and can be applied to various PCR reactions by adjusting the input parameters.

Q3: Is the efficiency percentage crucial for accurate results?

A: Yes, the efficiency percentage significantly impacts the final DNA concentration, so ensure to input it accurately.


In conclusion, the PCR cycle calculator presented here provides a handy solution for researchers and scientists involved in molecular biology experiments. By following the simple steps outlined above, you can make precise calculations tailored to your specific PCR reaction conditions.

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